pgi [2019-08-09 08:30:08]

glucose 6-phosphate isomerase, glycolytic / gluconeogenic enzyme

locus: BSU_31350

pI: 4.85

mw: 50.36 kDa

protein length: 451 aa

gene length: 1353 bp

function: enzyme in glycolysis / gluconeogenesis

product: glucose-6-phosphate isomerase

essential: no

ec: 5.3.1.9

synonyms: yugL

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.1|Glycolysis]
• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.2|Gluconeogenesis]
• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.9|Newly identified competence genes]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.6|Phosphorylation on a Thr residue]

Gene

Coordinates: 3,220,731 → 3,222,083

Phenotypes of a mutant

• poorly transformable [pubmed|28189581]

The protein

Catalyzed reaction/ biological activity

• D-glucose 6-phosphate = D-fructose 6-phosphate (according to Swiss-Prot) D-glucose 6-phosphate = D-fructose 6-phosphate

Protein family

• GPI family (single member, according to UniProt)

Modification

• phosphorylation on Thr-39 [Pubmed|17218307]
• phosphorylated on Arg-5 [Pubmed|31221751]

Effectors of protein activity

• competitively inhibited by 6-phosphogluconate (in ''B.caldotenax, B.stearothermophilus'') [Pubmed|7400125]

Structure

• [PDB|1B0Z] (''Geobacillus stearothermophilus''), [http://www.proteopedia.org/wiki/index.php/2pgi 2PGI] (''Geobacillus stearothermophilus'')

[SW|Localization]

• cytoplasm (homogeneously distributed throughout the cell) [Pubmed|24825009]

Additional information

• extensive information on the structure and enzymatic properties of Pgi can be found at [http://www.proteopedia.org/wiki/index.php/Phosphoglucose_isomerase Proteopedia]
• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|E3DAC5DE86EE0E34C6ECB6DE1E41309742465CF9|pgi]-[gene|BD63E58F9CF99F9A1888099F3393B30DE3F164F5|yugM]-[gene|0C8E5DFD40F4B2EB540199DFB2520E3FBC878530|yugN]
  description: [Pubmed|11489127]

  regulation

  • constitutively expressed [Pubmed|11489127]

Biological materials

Mutant

• GP508 (spc), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]
• GP1746: BSB1 ''pgi::aphA3'', available in [SW|Jörg Stülke]' lab
• BKE31350 (Δ[gene|E3DAC5DE86EE0E34C6ECB6DE1E41309742465CF9|pgi]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31350 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGCTTGTCCCTCCATAA, downstream forward: _UP4_TAATGTGAGAAAGCTGACTG
• BKK31350 (Δ[gene|E3DAC5DE86EE0E34C6ECB6DE1E41309742465CF9|pgi]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31350 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGCTTGTCCCTCCATAA, downstream forward: _UP4_TAATGTGAGAAAGCTGACTG

Expression vectors

• pGP398 (N-terminal His-tag, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

lacZ fusion

• pGP510 (in [SW|pAC6]), available in [SW|Jörg Stülke]'s lab

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab, [pubmed|19193632]

labs

• [SW|Jörg Stülke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html homepage]

References

• 17218307,19052382,4214896,23420519,11489127,4275311,11491085,17218307,24571712,15378759,24825009,28189581,31221751